Hotspot

trnL-UAA-trnF-GAA

LSC · 50123-50491 · 369 bp

Back to Melanosciadium

Pi 0.00921

MarkerSeek score 61.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 5
pi: 0.00921
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TCTACCAGCTGAGCTATCCC	45.0	55.0	57.8	58.0	475-482 bp	1.0	80.4
trnL-UAA-trnF-GAA_p2	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	511-518 bp	1.0	79.9
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	GTGCCAGGAACCAGATTTGA	45.0	50.0	57.8	58.1	526-533 bp	1.0	79.6
trnL-UAA-trnF-GAA_p4	ACATGTCAATACCGGCAACA	TCAGTCCTCTGCTCTACCAG	45.0	55.0	57.8	57.9	487-494 bp	1.0	79.3
trnL-UAA-trnF-GAA_p5	ACATGTCAATACCGGCAACA	CATGTGCCAGGAACCAGATT	45.0	50.0	57.8	58.2	529-536 bp	1.0	79.0