Hotspot

rps8

LSC · 79163-79567 · 405 bp

Back to Melampyrum

Pi 0.00642

MarkerSeek score 37.40

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 5
pi: 0.00642
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AAACTACGCCGGATCTTTCC	GTAGGTTGTGTCTCACGCAT	50.0	50.0	58.0	57.9	761-774 bp	1.0	84.1
rps8_p2	AAACTACGCCGGATCTTTCC	GGGCTCGAGAATTGATGTGT	50.0	50.0	58.0	58.0	1143-1162 bp	1.0	83.6
rps8_p3	ATTCCTACGCGAACCAGTTC	GGGCTCGAGAATTGATGTGT	50.0	50.0	58.0	58.0	1617-1639 bp	1.0	83.0
rps8_p4	ATTCCTACGCGAACCAGTTC	GTAGGTTGTGTCTCACGCAT	50.0	50.0	58.0	57.9	1234-1253 bp	1.0	82.9
rps8_p5	TACATTGAGCCCTTTCGGTG	GGGCTCGAGAATTGATGTGT	50.0	50.0	57.9	58.0	1503-1525 bp	1.0	82.4