Hotspot

rpl32-trnL-UAG

SSC · 108567-109301 · 735 bp

Back to Melampyrum

Pi 0.0242

MarkerSeek score 65.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 735
variable_sites: 35
pi: 0.0242
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGATATTGGACAGCGGTA	GGATTCGAACCGAGATGCTT	50.0	50.0	57.9	58.1	881-899 bp	0.8	76.6
rpl32-trnL-UAG_p2	GAAGGGATATTGGACAGCGG	GGATTCGAACCGAGATGCTT	55.0	50.0	58.1	58.1	883-901 bp	0.8	76.4
rpl32-trnL-UAG_p3	GGAAGGGATATTGGACAGCG	TGGAAAATGGCCCGAAGAAA	55.0	45.0	58.1	58.0	1208-1224 bp	0.8	76.3
rpl32-trnL-UAG_p4	AGGGATATTGGACAGCGGTA	TGGAAAATGGCCCGAAGAAA	50.0	45.0	57.9	58.0	1205-1221 bp	0.6	68.3
rpl32-trnL-UAG_p5	GAAGGGATATTGGACAGCGG	TGGAAAATGGCCCGAAGAAA	55.0	45.0	58.1	58.0	1207-1223 bp	0.6	68.2