Hotspot

accD-psaI

LSC · 58165-58799 · 635 bp

Back to Melampyrum

Pi 0.0181

MarkerSeek score 65.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 24
pi: 0.0181
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCGTACCGCGCAATCTTTTA	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	794-826 bp	1.0	82.2
accD-psaI_p2	TCGTACCGCGCAATCTTTTA	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	800-832 bp	1.0	81.6
accD-psaI_p3	TAATCGTACCGCGCAATCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.8	58.0	797-829 bp	1.0	80.5
accD-psaI_p4	CTAATCGTACCGCGCAATCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	798-830 bp	1.0	80.4
accD-psaI_p5	TAATCGTACCGCGCAATCTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	803-835 bp	1.0	79.9