Hotspot

rbcL-accD

LSC · 56150-56724 · 575 bp

Back to Melampyrum

Pi 0.0255

MarkerSeek score 67.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 575
variable_sites: 28
pi: 0.0255
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	TTTCTTGCCTGCTATTTGC	55.0	42.1	58.0	54.2	705-737 bp	1.0	46.6
rbcL-accD_p2	TTTGAGTTTCGCGCAGTAGA	TTTCTTGCCTGCTATTTGC	45.0	42.1	57.9	54.2	645-677 bp	1.0	46.2
rbcL-accD_p3	CGCGCAGTAGATACTTTGGA	TTTCTTGCCTGCTATTTGC	50.0	42.1	57.8	54.2	636-668 bp	1.0	45.8
rbcL-accD_p4	TCGCGCAGTAGATACTTTGG	TTTCTTGCCTGCTATTTGC	50.0	42.1	57.8	54.2	637-669 bp	1.0	45.8
rbcL-accD_p5	GCGAGGTCTGGAAAGAGATC	TTTCTTGCCTGCTATTTGC	55.0	42.1	57.8	54.2	668-700 bp	1.0	45.4