Hotspot

ycf3-trnS-GGA

LSC · 43783-44646 · 864 bp

Back to Melampyrum

Pi 0.0213

MarkerSeek score 65.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 36
pi: 0.0213
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	ACTTCACCTTCAACAAGCGT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	58.0	58.1	1126-1160 bp	1.0	85.3
ycf3-trnS-GGA_p2	ACTTCACCTTCAACAAGCGT	CTCAACTCTTCCCCTCATGC	45.0	55.0	58.0	58.0	1549-1588 bp	1.0	84.5
ycf3-trnS-GGA_p3	ACTTCACCTTCAACAAGCGT	TGGGTATGGCCTCGACTATT	45.0	50.0	58.0	57.9	1710-1749 bp	1.0	83.8
ycf3-trnS-GGA_p4	ACTTCACCTTCAACAAGCGT	TTGGGTATGGCCTCGACTAT	45.0	50.0	58.0	57.9	1711-1750 bp	1.0	83.8
ycf3-trnS-GGA_p5	TCCCGAATCATGCGTCTTTT	CTCAACTCTTCCCCTCATGC	45.0	55.0	57.9	58.0	1533-1537 bp	0.4	55.8