Hotspot

trnT-GGU-psbD

LSC · 30971-32263 · 1293 bp

Back to Melampyrum

Pi 0.0143

MarkerSeek score 63.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1293
variable_sites: 34
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.3	58.0	1384-1410 bp	1.0	82.4
trnT-GGU-psbD_p2	CCCCTTTAACTCAGCGGTAG	CCGCAACCAGTCATCCATAA	55.0	50.0	57.7	58.0	1420-1446 bp	1.0	82.0
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1414-1440 bp	1.0	81.6
trnT-GGU-psbD_p4	CCCCTTTAACTCAGCGGTAG	GGACCAGCCTACAAAAACGA	55.0	50.0	57.7	58.1	1450-1476 bp	1.0	81.3
trnT-GGU-psbD_p5	CGCTATGGTAAGGCGTAAGT	CCGCAACCAGTCATCCATAA	50.0	50.0	57.5	58.0	1395-1421 bp	1.0	80.5