Hotspot

atpI-rps2

LSC · 14864-15100 · 237 bp

Back to Melampyrum

Pi 0.0346

MarkerSeek score 68.40

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 237
variable_sites: 17
pi: 0.0346
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	CCTACCTCCACACCGGATAT	GGCTTTGCGAGAGTGTATCA	55.0	50.0	58.0	58.0	480-517 bp	1.0	84.8
atpI-rps2_p2	CCTACCTCCACACCGGATAT	TGGCTTTGCGAGAGTGTATC	55.0	50.0	58.0	58.0	481-518 bp	1.0	84.8
atpI-rps2_p3	CCTACCTCCACACCGGATAT	CCTAAAAGAGATGCGGCTGT	55.0	50.0	58.0	58.0	605-642 bp	1.0	83.8
atpI-rps2_p4	ACGTGAGTAAAGCCAAAGCA	GGCTTTGCGAGAGTGTATCA	45.0	50.0	58.0	58.0	845-882 bp	1.0	82.9
atpI-rps2_p5	ACGTGAGTAAAGCCAAAGCA	TGGCTTTGCGAGAGTGTATC	45.0	50.0	58.0	58.0	846-883 bp	1.0	82.8