Hotspot

trnS-GCU-trnG-UCC

LSC · 8117-8814 · 698 bp

Back to Melampyrum

Pi 0.0384

MarkerSeek score 82.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 53
pi: 0.0384
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.0	56.4	807-825 bp	1.0	75.4
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.0	56.4	806-824 bp	1.0	75.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.2	56.4	861-879 bp	1.0	73.7
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.0	57.1	808-826 bp	1.0	73.6
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.0	57.1	807-825 bp	1.0	73.6