Hotspot

rps16-trnQ-UUG

LSC · 6163-6875 · 713 bp

Back to Melampyrum

Pi 0.0534
MarkerSeek score 84.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
713
variable_sites
65
pi
0.0534
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GGTTCGAACCCTTCCGTC 45.0 61.1 58.1 57.7 779-819 bp 1.0 71.2
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTTCGAACCCTTCCGTCC 45.0 61.1 58.1 57.7 778-818 bp 1.0 71.2
rps16-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG GGTTCGAACCCTTCCGTC 50.0 61.1 57.2 57.7 771-811 bp 1.0 66.1
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GTTCGAACCCTTCCGTCC 50.0 61.1 57.2 57.7 770-810 bp 1.0 66.1
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGAACCCTTCCGTCCC 45.0 61.1 58.1 58.9 777-817 bp 1.0 65.9