Hotspot

trnH-GUG-psbA

LSC · 86-558 · 473 bp

Back to Melampyrum

Pi 0.0486

MarkerSeek score 65.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 36
pi: 0.0486
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTGGCTGCTAT	55.0	55.0	58.1	58.0	401-536 bp	1.0	89.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	415-550 bp	1.0	89.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	414-549 bp	1.0	89.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTGGCTGCTAT	50.0	55.0	57.9	58.0	416-551 bp	1.0	89.0
trnH-GUG-psbA_p5	GCCTTGATCCACTTGGCTAC	CCTCTAGACCTGGCTGCTAT	55.0	55.0	58.6	58.0	406-541 bp	1.0	85.2