Hotspot

trnR-ACG-rrn5

IRa · 135867-136112 · 246 bp

Back to Melaleuca

Pi 0.0328

MarkerSeek score 76.90

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: IRa
length_bp: 246
variable_sites: 12
pi: 0.0328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	CCACGTGCTCTAATCCTCTG	GGTGACGATACTGTAGGGGA	55.0	55.0	57.8	57.9	319-330 bp	1.0	83.4
trnR-ACG-rrn5_p2	CCACGTGCTCTAATCCTCTG	AATCCATCCCGAACTTGGTG	55.0	50.0	57.8	57.9	354-365 bp	1.0	82.3
trnR-ACG-rrn5_p3	CCACGTGCTCTAATCCTCTG	ACCAATCCATCCCGAACTTG	55.0	50.0	57.8	57.9	357-368 bp	1.0	82.3
trnR-ACG-rrn5_p4	CCACGTGCTCTAATCCTCTG	CAATCCATCCCGAACTTGGT	55.0	50.0	57.8	57.9	355-366 bp	1.0	82.3
trnR-ACG-rrn5_p5	CCACGTGCTCTAATCCTCTG	CACCAATCCATCCCGAACTT	55.0	50.0	57.8	57.9	358-369 bp	1.0	82.3