Hotspot

psbT-psbN

LSC · 78140-78204 · 65 bp

Back to Melaleuca

Pi 0.0308

MarkerSeek score 69.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 3
pi: 0.0308
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	ATCCATTCGAAGAACACGGG	47.6	50.0	57.6	58.0	130 bp	1.0	70.5
psbT-psbN_p2	TCGAGAACCACCTAAAGTTCC	ATCCATTCGAAGAACACGGG	47.6	50.0	57.3	58.0	131 bp	1.0	68.3
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	TTCGAAGAACACGGGGACTA	47.6	50.0	57.6	58.4	125 bp	1.0	67.7
psbT-psbN_p4	TTCGAGAACCACCTAAAGTTCC	ATCCATTCGAAGAACACGGG	45.5	50.0	58.0	58.0	132 bp	1.0	65.6
psbT-psbN_p5	TCGAGAACCACCTAAAGTTCC	TTCGAAGAACACGGGGACTA	47.6	50.0	57.3	58.4	126 bp	1.0	65.5