Hotspot

rps18-rpl20

LSC · 72229-72502 · 274 bp

Back to Melaleuca

Pi 0.0487

MarkerSeek score 82.00

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 274
variable_sites: 16
pi: 0.0487
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TTGAAAGAAGCGAGTCGACC	TCTGTACAAGAGACAGTTGCT	50.0	42.9	58.2	56.6	431-444 bp	1.0	65.4
rps18-rpl20_p2	TTGAAAGAAGCGAGTCGACC	TCTGTACAAGAGACAGTTGCTT	50.0	40.9	58.2	57.3	431-444 bp	1.0	63.1
rps18-rpl20_p3	TTGAAAGAAGCGAGTCGACC	TGTACAAGAGACAGTTGCTTCT	50.0	40.9	58.2	57.3	429-442 bp	1.0	63.1
rps18-rpl20_p4	TTGAAAGAAGCGAGTCGACC	ATCTGTACAAGAGACAGTTGCT	50.0	40.9	58.2	57.1	432-445 bp	1.0	61.4
rps18-rpl20_p5	TTGAAAGAAGCGAGTCGACC	TCTGTACAAGAGACAGTTGC	50.0	45.0	58.2	55.1	431-444 bp	1.0	60.9