Hotspot

trnT-GGU-psbD

LSC · 34167-35571 · 1405 bp

Back to Melaleuca

Pi 0.0182

MarkerSeek score 67.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1405
variable_sites: 36
pi: 0.0182
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACGGTCTCTACGTAACCAGT	50.0	50.0	57.9	57.8	1491-1558 bp	1.0	83.5
trnT-GGU-psbD_p2	ATGGGCCCTTTTAACTCAGC	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.1	57.8	1495-1562 bp	1.0	83.2
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	50.0	50.0	57.9	57.7	1516-1583 bp	1.0	82.9
trnT-GGU-psbD_p4	ATGGGCCCTTTTAACTCAGC	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1520-1587 bp	1.0	82.6
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	TAGACCGGACCAACCTACAA	50.0	50.0	57.9	57.7	1518-1585 bp	1.0	82.6