Hotspot

trnD-GUC-trnY-GUA

LSC · 32547-32992 · 446 bp

Back to Melaleuca

Pi 0.0867

MarkerSeek score 83.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 44
pi: 0.0867
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTTGATTCCGTCGGGACTG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	414-599 bp	1.0	88.6
trnD-GUC-trnY-GUA_p2	ATTTGATTCCGTCGGGACTG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	413-598 bp	1.0	88.6
trnD-GUC-trnY-GUA_p3	ATTTGATTCCGTCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	435-620 bp	1.0	87.4
trnD-GUC-trnY-GUA_p4	ATTTGATTCCGTCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	434-619 bp	1.0	87.2
trnD-GUC-trnY-GUA_p5	ATTTGATTCCGTCGGGACTG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.0	58.4	468-653 bp	1.0	86.6