Hotspot

trnC-GCA-petN

LSC · 29357-30235 · 879 bp

Back to Melaleuca

Pi 0.0556

MarkerSeek score 84.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 58
pi: 0.0556
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	793-976 bp	1.0	88.4
trnC-GCA-petN_p2	CATACATTTTGGCGGCATGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	845-1028 bp	1.0	87.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	787-970 bp	1.0	87.0
trnC-GCA-petN_p4	CATACATTTTGGCGGCATGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	839-1022 bp	1.0	86.2
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	811-994 bp	1.0	86.1