Hotspot

psbI-trnS-GCU

LSC · 8640-8792 · 153 bp

Back to Melaleuca

Pi 0.0544

MarkerSeek score 74.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 12
pi: 0.0544
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	749-768 bp	1.0	85.5
psbI-trnS-GCU_p2	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	750-769 bp	1.0	85.5
psbI-trnS-GCU_p3	GTTTGGCAAGCTGCTGTAAG	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.9	58.1	768-787 bp	1.0	84.5
psbI-trnS-GCU_p4	GTTTGGCAAGCTGCTGTAAG	TTTTTCGTACCGAGGGTTCG	50.0	50.0	57.9	58.2	712-731 bp	1.0	84.1
psbI-trnS-GCU_p5	TGGCAAGCTGCTGTAAGTTT	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.3	58.0	746-765 bp	1.0	83.5