Hotspot

ycf1

SSC · 123759-129095 · 5337 bp

Back to Megacarpaea

Pi 0.0164

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5337
variable_sites: 201
pi: 0.0164
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCATCGCAATTGAAAACGC	TTACCTCAGATTGCATGGGC	45.0	50.0	58.0	57.9	964-988 bp	1.0	84.9
ycf1_p2	TCCATCGCAATTGAAAACGC	CCTCAGATTGCATGGGCTAG	45.0	55.0	58.0	58.1	961-985 bp	1.0	84.5
ycf1_p3	ACAGACAAACGGACAGGTTC	TTACCTCAGATTGCATGGGC	50.0	50.0	58.1	57.9	1166-1190 bp	1.0	84.4
ycf1_p4	CAGACAAACGGACAGGTTCA	TTACCTCAGATTGCATGGGC	50.0	50.0	58.1	57.9	1165-1189 bp	1.0	84.4
ycf1_p5	ACAGACAAACGGACAGGTTC	CCTCAGATTGCATGGGCTAG	50.0	55.0	58.1	58.1	1163-1187 bp	1.0	84.0