Hotspot

ndhE-ndhG

SSC · 117967-118227 · 261 bp

Back to Megacarpaea

Pi 0.0208

MarkerSeek score 64.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 261
variable_sites: 12
pi: 0.0208
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGACTCGTGATCAATCCA	TAGGTGCAATTTCTGTGGCT	45.0	45.0	56.7	57.4	362-367 bp	1.0	69.0
ndhE-ndhG_p2	TTCGACTCGTGATCAATCCA	AGGTGCAATTTCTGTGGCTC	45.0	50.0	56.7	58.7	361-366 bp	1.0	67.7
ndhE-ndhG_p3	TCGACTCGTGATCAATCCAT	TAGGTGCAATTTCTGTGGCT	45.0	45.0	56.5	57.4	361-366 bp	1.0	67.1
ndhE-ndhG_p4	TTTCGACTCGTGATCAATCCA	TAGGTGCAATTTCTGTGGCT	42.9	45.0	57.4	57.4	363-368 bp	1.0	66.8
ndhE-ndhG_p5	TCGACTCGTGATCAATCCAT	AGGTGCAATTTCTGTGGCTC	45.0	50.0	56.5	58.7	360-365 bp	1.0	65.8