Hotspot

trnW-trnP

LSC · 65710-65895 · 186 bp

Back to Megacarpaea

Pi 0.043

MarkerSeek score 68.80

Primer available yes

Label trnW-trnP

Feature Metadata

label_name: trnW-trnP
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 15
pi: 0.043
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-trnP_p1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	267-276 bp	1.0	84.3
trnW-trnP_p2	GTTTTGGAGACCCACGTTCT	ACGTAACCCACCCCATTTTC	50.0	50.0	58.0	58.1	542-553 bp	1.0	83.9
trnW-trnP_p3	GTTTTGGAGACCCACGTTCT	CGTAACCCACCCCATTTTCA	50.0	50.0	58.0	58.1	541-552 bp	1.0	83.9
trnW-trnP_p4	GCGGAAGGTCCAATTCAGAT	ACGTAACCCACCCCATTTTC	50.0	50.0	58.0	58.1	660 bp	0.2	47.0
trnW-trnP_p5	GCGGAAGGTCCAATTCAGAT	CGTAACCCACCCCATTTTCA	50.0	50.0	58.0	58.1	659 bp	0.2	46.9