Hotspot

petA-psbJ

LSC · 62333-63368 · 1036 bp

Back to Megacarpaea

Pi 0.0242

MarkerSeek score 65.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1036
variable_sites: 52
pi: 0.0242
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1675-1744 bp	1.0	86.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1674-1743 bp	1.0	86.2
petA-psbJ_p3	GTTTCAGAGGGCGAATCCAT	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1771-1840 bp	1.0	86.1
petA-psbJ_p4	AGTGGATGCATCGAATGGAC	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1833-1902 bp	1.0	85.9
petA-psbJ_p5	AGTGGATGCATCGAATGGAC	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1832-1901 bp	1.0	85.9