Hotspot

psbM-trnD

LSC · 28733-29788 · 1056 bp

Back to Megacarpaea

Pi 0.0235

MarkerSeek score 66.90

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 50
pi: 0.0235
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AGAATGAAGAGTGCAGTAGC	AATTGGTTAGAGCACCGCC	45.0	52.6	55.2	58.1	1139-1166 bp	1.0	54.3
psbM-trnD_p2	AGAATGAAGAGTGCAGTAGC	ATTGGTTAGAGCACCGCC	45.0	55.6	55.2	57.4	1138-1165 bp	1.0	45.9
psbM-trnD_p3	AGAATGAAGAGTGCAGTAGC	AATTGGTTAGAGCACCGCCC	45.0	55.0	55.2	60.7	1139-1166 bp	1.0	45.9
psbM-trnD_p4	AGAATGAAGAGTGCAGTAGC	TTAGAGCACCGCCCTGTC	45.0	61.1	55.2	59.0	1133-1160 bp	1.0	45.9
psbM-trnD_p5	AGAATGAAGAGTGCAGTAGC	ATTGGTTAGAGCACCGCCC	45.0	57.9	55.2	60.1	1138-1165 bp	1.0	45.9