Hotspot

trnR-atpA

LSC · 9473-9772 · 300 bp

Back to Megacarpaea

Pi 0.0282

MarkerSeek score 67.90

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 300
variable_sites: 17
pi: 0.0282
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	GGTTCAAATCCTATTGGACGC	TCAAGAGCAACTGGAACGTT	47.6	45.0	57.3	57.7	349-365 bp	1.0	71.7
trnR-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCAAGAGCAACTGGAACGT	47.6	45.0	57.3	57.7	350-366 bp	1.0	71.6
trnR-atpA_p3	CGAAAAGCGTCCATTGTCTA	TCAAGAGCAACTGGAACGTT	45.0	45.0	56.2	57.7	405-421 bp	1.0	70.1
trnR-atpA_p4	CGAAAAGCGTCCATTGTCTA	TTCAAGAGCAACTGGAACGT	45.0	45.0	56.2	57.7	406-422 bp	1.0	70.1
trnR-atpA_p5	CGAAAAGCGTCCATTGTCTAA	TCAAGAGCAACTGGAACGTT	42.9	45.0	56.9	57.7	405-421 bp	1.0	68.1