Hotspot

trnT-trnR

LSC · 9241-9400 · 160 bp

Back to Megacarpaea

Pi 0.027

MarkerSeek score 69.10

Primer available yes

Label trnT-trnR

Feature Metadata

label_name: trnT-trnR
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 9
pi: 0.027
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnR_p1	AGCCTTCCAAGCTAACGATG	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	271-283 bp	1.0	53.7
trnT-trnR_p2	ACCCTTAGCCTTCCAAGCTA	TGATATTGCGTCCAATAGGA	50.0	40.0	58.0	53.9	277-289 bp	1.0	53.6
trnT-trnR_p3	AAACCCTTAGCCTTCCAAGC	TGATATTGCGTCCAATAGGA	50.0	40.0	58.1	53.9	279-291 bp	1.0	53.2
trnT-trnR_p4	CGACGTCGACTAAAACCCTT	TGATATTGCGTCCAATAGGA	50.0	40.0	57.9	53.9	291-303 bp	1.0	53.1
trnT-trnR_p5	AGCCTTCCAAGCTAACGATG	TTGATATTGCGTCCAATAGGA	50.0	38.1	58.0	54.8	272-284 bp	1.0	52.7