Hotspot

trnK-trnQ

LSC · 4323-6647 · 2325 bp

Back to Megacarpaea

Pi 0.0198

MarkerSeek score 61.30

Primer available yes

Label trnK-trnQ

Feature Metadata

label_name: trnK-trnQ
feature_type: igs
region: LSC
length_bp: 2325
variable_sites: 102
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-trnQ_p1	CCTTTCAGGATCAGTCGTGG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	2434-2478 bp	1.0	85.3
trnK-trnQ_p2	TCAGGATCAGTCGTGGTCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	2430-2474 bp	1.0	84.9
trnK-trnQ_p3	TTCAGGATCAGTCGTGGTCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	2431-2475 bp	1.0	84.9
trnK-trnQ_p4	AACTCTACCGCAGATCTGGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	2407-2451 bp	0.8	75.6
trnK-trnQ_p5	AAGCCGAGTACTCTACCGTT	CTTACAGCTGCTTGCCAAAC	50.0	50.0	58.2	57.9	2902-2946 bp	0.8	74.3