Hotspot

rps11

LSC · 80211-80627 · 417 bp

Back to Meeboldia

Pi 0.0024

MarkerSeek score 36.10

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 2
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	ACTTCCACTGTAGTGTCCGA	GGATATATCCATATATCTCTGACTCA	50.0	34.6	58.0	54.9	579-588 bp	1.0	40.3
rps11_p2	TTCCACTGTAGTGTCCGAGT	GGATATATCCATATATCTCTGACTCA	50.0	34.6	58.0	54.9	577-586 bp	1.0	40.3
rps11_p3	CACTTCCACTGTAGTGTCCG	GGATATATCCATATATCTCTGACTCA	55.0	34.6	57.9	54.9	580-589 bp	1.0	40.3
rps11_p4	ACTTCCACTGTAGTGTCCGA	GGATATATCCATATATCTCTGACTCAT	50.0	33.3	58.0	55.4	579-588 bp	1.0	40.3
rps11_p5	TTCCACTGTAGTGTCCGAGT	GGATATATCCATATATCTCTGACTCAT	50.0	33.3	58.0	55.4	577-586 bp	1.0	40.3