Hotspot

psbA

LSC · 260-1321 · 1062 bp

Back to Meeboldia

Pi 0.000942

MarkerSeek score 39.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.000942
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACTCGTATAGACTAAGACCCCA	AAATTCGTGTGCTTGGGAGT	45.5	45.0	57.2	58.0	1137 bp	1.0	57.7
psbA_p2	ACTCGTATAGACTAAGACCCCA	TGCTTGGGAGTCCCTGATAA	45.5	50.0	57.2	58.0	1128 bp	1.0	57.6
psbA_p3	AACTCGTATAGACTAAGACCCCA	AAATTCGTGTGCTTGGGAGT	43.5	45.0	57.9	58.0	1138 bp	1.0	55.0
psbA_p4	AACTCGTATAGACTAAGACCCCA	TGCTTGGGAGTCCCTGATAA	43.5	50.0	57.9	58.0	1129 bp	1.0	54.9
psbA_p5	ACTCGTATAGACTAAGACCCCA	AATTCGTGTGCTTGGGAGTC	45.5	50.0	57.2	58.5	1136 bp	1.0	54.0