Hotspot

ndhI

SSC · 120879-121382 · 504 bp

Back to Meeboldia

Pi 0.00231

MarkerSeek score 35.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 2
pi: 0.00231
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AAACCATCGACGTAACTCCG	TTTGTGACCTTTGAGGAGCC	50.0	50.0	58.0	58.0	1914-1928 bp	1.0	80.4
ndhI_p2	AAGACCCGATCCCAGAAAGA	TTTGTGACCTTTGAGGAGCC	50.0	50.0	58.0	58.0	1661-1675 bp	1.0	80.1
ndhI_p3	AAACCATCGACGTAACTCCG	GACCTTTGAGGAGCCGTATG	50.0	55.0	58.0	58.1	1909-1923 bp	1.0	80.1
ndhI_p4	AAGACCCGATCCCAGAAAGA	GACCTTTGAGGAGCCGTATG	50.0	55.0	58.0	58.1	1656-1670 bp	1.0	79.8
ndhI_p5	AATACCACTCCCAGACCTCC	TTTGTGACCTTTGAGGAGCC	55.0	50.0	58.1	58.0	1687-1701 bp	1.0	79.6