Hotspot

ndhF

SSC · 111713-113944 · 2232 bp

Back to Meeboldia

Pi 0.00314

MarkerSeek score 45.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 14
pi: 0.00314
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCTCATGAAGGCCTACCATT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.6	58.3	2350-2366 bp	1.0	76.1
ndhF_p2	TGAAGGCCTACCATTCGAGT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.4	58.3	2345-2361 bp	1.0	75.5
ndhF_p3	AGCTCATGAAGGCCTACCAT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2351-2367 bp	1.0	75.1
ndhF_p4	AATTGGAATTCCTGTCGGGC	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2373-2389 bp	1.0	74.7
ndhF_p5	CATGAAGGCCTACCATTCGA	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.4	58.3	2347-2363 bp	1.0	74.0