Hotspot

ndhF-rpl32

SSC · 113945-114902 · 958 bp

Back to Meeboldia

Pi 0.00697

MarkerSeek score 67.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 958
variable_sites: 13
pi: 0.00697
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	GCTGCCCAATATCCCTTACC	50.0	55.0	58.0	58.1	1058-1151 bp	1.0	84.0
ndhF-rpl32_p2	GTTGCCGACGGAAAAAGAAG	GGCTGCCCAATATCCCTTAC	50.0	55.0	58.0	58.1	1059-1152 bp	1.0	84.0
ndhF-rpl32_p3	TGCCGACGGAAAAAGAAGAA	GCTGCCCAATATCCCTTACC	45.0	55.0	58.0	58.1	1056-1149 bp	1.0	83.9
ndhF-rpl32_p4	TGCCGACGGAAAAAGAAGAA	GGCTGCCCAATATCCCTTAC	45.0	55.0	58.0	58.1	1057-1150 bp	1.0	83.9
ndhF-rpl32_p5	TTGCCGACGGAAAAAGAAGA	GCTGCCCAATATCCCTTACC	45.0	55.0	58.0	58.1	1057-1150 bp	1.0	83.9