Hotspot

rpl33-rps18

LSC · 69014-69224 · 211 bp

Back to Meeboldia

Pi 0.0142

MarkerSeek score 65.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 211
variable_sites: 6
pi: 0.0142
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AATCGTCACAACACGCCTAA	GGTCGCTTGGGTTTATCCAT	45.0	50.0	57.8	57.9	316-357 bp	1.0	81.0
rpl33-rps18_p2	AATCGTCACAACACGCCTAA	AAGAAAAGGTCGCTTGGGTT	45.0	45.0	57.8	57.9	323-364 bp	1.0	80.8
rpl33-rps18_p3	AATCGTCACAACACGCCTAA	AGAAAAGGTCGCTTGGGTTT	45.0	45.0	57.8	57.9	322-363 bp	1.0	80.8
rpl33-rps18_p4	AATCGTCACAACACGCCTAA	AAGGTCGCTTGGGTTTATCC	45.0	50.0	57.8	57.9	318-359 bp	1.0	80.4
rpl33-rps18_p5	GTCACAACACGCCTAATCGA	GGTCGCTTGGGTTTATCCAT	50.0	50.0	58.3	57.9	312-353 bp	1.0	79.9