Hotspot

ycf4-cemA

LSC · 61650-62293 · 644 bp

Back to Meeboldia

Pi 0.00543

MarkerSeek score 66.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 644
variable_sites: 7
pi: 0.00543
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACTCGTACGG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.1	58.1	676-821 bp	1.0	83.5
ycf4-cemA_p2	CCATTCCCTTGACTCGTACG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.1	58.1	677-822 bp	1.0	83.5
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	612-757 bp	1.0	83.4
ycf4-cemA_p4	ATTCCCTTGACTCGTACGGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.2	58.1	675-820 bp	1.0	82.9
ycf4-cemA_p5	TTCCCTTGACTCGTACGGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.2	58.1	674-819 bp	1.0	82.9