Hotspot

trnE-UUC-trnT-GGU

LSC · 31718-32437 · 720 bp

Back to Meeboldia

Pi 0.0125

MarkerSeek score 55.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 16
pi: 0.0125
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	372-797 bp	1.0	83.7
trnE-UUC-trnT-GGU_p2	TTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	57.4	57.9	407-832 bp	1.0	80.5
trnE-UUC-trnT-GGU_p3	CCGTTGCCTCCTTGAAAGAG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.8	57.9	373-798 bp	1.0	78.3
trnE-UUC-trnT-GGU_p4	TTTGTAGTACCCTACCCCCA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.0	57.9	408-833 bp	1.0	76.6
trnE-UUC-trnT-GGU_p5	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	59.1	57.9	344-769 bp	1.0	76.4