Hotspot

ycf1

SSC · 125111-130531 · 5421 bp

Back to Medinilla

Pi 0.014

MarkerSeek score 54.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5421
variable_sites: 163
pi: 0.014
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATTGGAGAAGGAATTCGA	CGGATTCAATGGTATCGATTAGT	40.0	39.1	54.1	56.3	167 bp	1.0	45.3
ycf1_p2	TCATTGGAGAAGGAATTCGA	CGGATTCAATGGTATCGATT	40.0	40.0	54.1	53.3	167 bp	1.0	45.3
ycf1_p3	TTCATTGGAGAAGGAATTCGA	CGGATTCAATGGTATCGATTAGT	38.1	39.1	54.9	56.3	168 bp	1.0	45.2
ycf1_p4	TTCATTGGAGAAGGAATTCGA	CGGATTCAATGGTATCGATT	38.1	40.0	54.9	53.3	168 bp	1.0	45.2
ycf1_p5	TTTCATTGGAGAAGGAATTCGA	CGGATTCAATGGTATCGATTAGT	36.4	39.1	55.7	56.3	169 bp	1.0	45.2