Hotspot

rpl22

LSC · 84648-85085 · 438 bp

Back to Medinilla

Pi 0.0381

MarkerSeek score 70.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 438
variable_sites: 30
pi: 0.0381
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTGTCCCATAATCCCCCACT	CGTCGCCGTAGTAAATAGGG	50.0	55.0	58.0	58.0	935-964 bp	1.0	85.1
rpl22_p2	TTGTCCCATAATCCCCCACT	GTTGGACAAGTGGGGAATGT	50.0	50.0	58.0	58.0	1175-1204 bp	1.0	84.4
rpl22_p3	TTGTCCCATAATCCCCCACT	TGGACAAGTGGGGAATGTTG	50.0	50.0	58.0	58.0	1173-1202 bp	1.0	84.4
rpl22_p4	TCTATGCGTGCAATTCCCTC	GTTGGACAAGTGGGGAATGT	50.0	50.0	58.0	58.0	1357-1386 bp	1.0	83.6
rpl22_p5	TCTATGCGTGCAATTCCCTC	TGGACAAGTGGGGAATGTTG	50.0	50.0	58.0	58.0	1355-1384 bp	1.0	83.6