Hotspot

psbA

LSC · 305-1366 · 1062 bp

Back to Medinilla

Pi 0.00461

MarkerSeek score 49.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 10
pi: 0.00461
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAACTCGTCTAAATTCAGACT	TGGGAGTCCCTGATGATGAA	33.3	50.0	52.6	57.7	1133 bp	1.0	40.7
psbA_p2	AAACTCGTCTAAATTCAGACT	TTGGGAGTCCCTGATGATGA	33.3	50.0	52.6	57.7	1134 bp	1.0	40.7
psbA_p3	AAAACTCGTCTAAATTCAGACT	TGGGAGTCCCTGATGATGAA	31.8	50.0	53.4	57.7	1134 bp	1.0	40.7
psbA_p4	AAAACTCGTCTAAATTCAGACT	TTGGGAGTCCCTGATGATGA	31.8	50.0	53.4	57.7	1135 bp	1.0	40.7
psbA_p5	AAAAACTCGTCTAAATTCAGACT	TGGGAGTCCCTGATGATGAA	30.4	50.0	54.3	57.7	1135 bp	1.0	40.7