Hotspot

ndhF-rpl32

SSC · 114385-115005 · 621 bp

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Pi 0.153
MarkerSeek score 87.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
621
variable_sites
163
pi
0.153
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAAGTGGAATGAGAGGT TCCAAATATTCTTACGAATATGTT 50.0 25.0 58.0 52.3 725-833 bp 1.0 52.0
ndhF-rpl32_p2 TGGGAAGTGGAATGAGAGGT TTCCAAATATTCTTACGAATATGT 50.0 25.0 58.0 52.3 726-834 bp 1.0 52.0
ndhF-rpl32_p3 TGGGAAGTGGAATGAGAGGTA TCCAAATATTCTTACGAATATGTT 47.6 25.0 57.8 52.3 725-833 bp 1.0 52.0
ndhF-rpl32_p4 TGGGAAGTGGAATGAGAGGTA TTCCAAATATTCTTACGAATATGT 47.6 25.0 57.8 52.3 726-834 bp 1.0 52.0
ndhF-rpl32_p5 ATGGGAAGTGGAATGAGAGG TCCAAATATTCTTACGAATATGTT 50.0 25.0 56.6 52.3 726-834 bp 1.0 52.0