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rps8-rpl14

LSC · 81535-81825 · 291 bp

Back to Medinilla

Pi 0.0353

MarkerSeek score 71.30

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 291
variable_sites: 23
pi: 0.0353
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CAGCAATAGTGTCTCTACCCA	CGAGAATTGAGACAGTTAAATTTCAC	47.6	34.6	56.8	57.4	373-488 bp	1.0	47.2
rps8-rpl14_p2	ACGTCAGCAATAGTGTCTCT	CGAGAATTGAGACAGTTAAATTTCAC	45.0	34.6	56.3	57.4	377-492 bp	1.0	47.1
rps8-rpl14_p3	ACGTCAGCAATAGTGTCTCT	CGAGAATTGAGACAGTTAAATTTCA	45.0	32.0	56.3	56.2	377-492 bp	1.0	47.1
rps8-rpl14_p4	GTCAGCAATAGTGTCTCTACCC	CGAGAATTGAGACAGTTAAATTTCAC	50.0	34.6	58.0	57.4	375-490 bp	1.0	47.1
rps8-rpl14_p5	GTCAGCAATAGTGTCTCTACCC	CGAGAATTGAGACAGTTAAATTTCA	50.0	32.0	58.0	56.2	375-490 bp	1.0	47.1