Hotspot

petL-petG

LSC · 66604-66791 · 188 bp

Back to Medinilla

Pi 0.033

MarkerSeek score 68.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 13
pi: 0.033
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	GCAGCTTTAACTATAACCTCAGC	GGTCTAACTGATCACCACGT	43.5	50.0	57.6	57.0	356-358 bp	1.0	47.6
petL-petG_p2	GCAGCTTTAACTATAACCTCAGC	GGTCTAACTGATCACCACGTC	43.5	52.4	57.6	58.1	356-358 bp	1.0	46.9
petL-petG_p3	GCAGCTTTAACTATAACCTCAGC	AGGTCTAACTGATCACCACG	43.5	50.0	57.6	56.7	357-359 bp	1.0	45.2
petL-petG_p4	TGGTTTTCTACTAGCAGCTT	GGTCTAACTGATCACCACGT	40.0	50.0	54.3	57.0	369-371 bp	1.0	45.1
petL-petG_p5	TTGGTTTTCTACTAGCAGCT	GGTCTAACTGATCACCACGT	40.0	50.0	54.3	57.0	370-372 bp	1.0	45.0