Hotspot

psbZ-trnG-UCC

LSC · 36840-37141 · 302 bp

Back to Medinilla

Pi 0.0359
MarkerSeek score 70.30
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
302
variable_sites
21
pi
0.0359
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 GGTGGCTATCCTTAATTCTCTCA CGTCTTCTCCTTGGCAAAGA 43.5 50.0 57.4 57.8 380-416 bp 1.0 58.5
psbZ-trnG-UCC_p2 TGGTGGCTATCCTTAATTCTCTC CGTCTTCTCCTTGGCAAAGA 43.5 50.0 57.4 57.8 381-417 bp 1.0 58.5
psbZ-trnG-UCC_p3 TGGTGGCTATCCTTAATTCTCT CGTCTTCTCCTTGGCAAAGA 40.9 50.0 56.3 57.8 381-417 bp 1.0 57.4
psbZ-trnG-UCC_p4 GGTGGCTATCCTTAATTCTCTCAT CGTCTTCTCCTTGGCAAAGA 41.7 50.0 57.9 57.8 380-416 bp 1.0 53.9
psbZ-trnG-UCC_p5 TGGTGGCTATCCTTAATTCTC CGTCTTCTCCTTGGCAAAGA 42.9 50.0 54.8 57.8 381-417 bp 1.0 53.1