Hotspot

trnS-GCU-trnR-UCU

LSC · 7896-9693 · 1798 bp

Back to Medinilla

Pi 0.0207
MarkerSeek score 69.40
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1798
variable_sites
75
pi
0.0207
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1847-1912 bp 1.0 75.6
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1846-1911 bp 1.0 75.6
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1848-1913 bp 1.0 73.6
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1847-1912 bp 1.0 73.6
trnS-GCU-trnR-UCU_p5 AGCAATCCGACGCTTTAGTC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.4 56.6 1849-1914 bp 1.0 72.8