Hotspot

trnH-GUG-psbA

LSC · 76-304 · 229 bp

Back to Medinilla

Pi 0.0721
MarkerSeek score 77.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
229
variable_sites
30
pi
0.0721
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 344-355 bp 1.0 88.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CCCTCTAGACCTAGCTGCAT 55.0 55.0 58.1 58.0 297 bp 0.2 46.9
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CCCTCTAGACCTAGCTGCAT 50.0 55.0 57.9 58.0 311 bp 0.2 46.9
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC CCCTCTAGACCTAGCTGCAT 50.0 55.0 57.9 58.0 310 bp 0.2 46.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC CCCTCTAGACCTAGCTGCAT 50.0 55.0 57.9 58.0 312 bp 0.2 46.9