Hotspot

clpP

Genome · 68855-70073 · 1219 bp

Back to Medicago

Pi 0.0421

MarkerSeek score 76.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: Genome
length_bp: 1219
variable_sites: 270
pi: 0.0421
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AAAACCCATATAGACGGCGG	AACAGGGTCAGAGGGATCAA	50.0	50.0	57.8	58.0	2044 bp	0.047619	39.5
clpP_p2	AAAACCCATATAGACGGCGG	ACAGGGTCAGAGGGATCAAA	50.0	50.0	57.8	58.0	2043 bp	0.047619	39.5
clpP_p3	AAAACCCATATAGACGGCGG	ACGAACATACCCTGTCTCCA	50.0	50.0	57.8	58.1	2076 bp	0.047619	39.4
clpP_p4	AAAACCCATATAGACGGCGG	TGGACAGAAAGTAAACGGCC	50.0	50.0	57.8	58.1	1959 bp	0.047619	39.0
clpP_p5	AAAACCCATATAGACGGCGG	AGAACAGGGTCAGAGGGATC	50.0	55.0	57.8	58.1	2046 bp	0.047619	38.9