Hotspot

atpA-trnR-UCU

Genome · 51488-51691 · 204 bp

Back to Medicago

Pi 0.0501

MarkerSeek score 76.70

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: Genome
length_bp: 204
variable_sites: 61
pi: 0.0501
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TCAGGAACAGATGGAACTCT	GGTTCAAATCCTATTGGACGC	45.0	47.6	55.5	57.3	232-289 bp	0.952381	56.3
atpA-trnR-UCU_p2	AGCTATTCAGGAACAGATGGA	GGTTCAAATCCTATTGGACGC	42.9	47.6	56.1	57.3	221-295 bp	1.0	55.5
atpA-trnR-UCU_p3	TCAGGAACAGATGGAACTCTT	GGTTCAAATCCTATTGGACGC	42.9	47.6	56.3	57.3	232-289 bp	0.952381	54.8
atpA-trnR-UCU_p4	TTCAGGAACAGATGGAACTCT	GGTTCAAATCCTATTGGACGC	42.9	47.6	56.3	57.3	233-290 bp	0.952381	54.8
atpA-trnR-UCU_p5	TCAGGAACAGATGGAACTCT	TGGATAGGACAGAGGTCTTCT	45.0	47.6	55.5	56.9	267-324 bp	0.952381	53.4