Hotspot

psbM-petN

Genome · 31630-32293 · 664 bp

Back to Medicago

Pi 0.0253

MarkerSeek score 78.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 664
variable_sites: 91
pi: 0.0253
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AGCTACTGCACTCTTCATTCT	AGTAAGTCTTGCTTGGGCTG	42.9	50.0	56.7	57.8	633-1077 bp	1.0	71.9
psbM-petN_p2	TCGCATTTATAGCTACTGCACT	AGTAAGTCTTGCTTGGGCTG	40.9	50.0	57.7	57.8	643-1087 bp	1.0	71.3
psbM-petN_p3	AGCTACTGCACTCTTCATTCT	ATGGGGAAGAAGTGGACTCT	42.9	50.0	56.7	57.6	573-1017 bp	1.0	71.0
psbM-petN_p4	AGCTACTGCACTCTTCATTCT	TCTTGCTTGGGCTGGTTTAA	42.9	45.0	56.7	57.6	627-1071 bp	1.0	70.5
psbM-petN_p5	AGCTACTGCACTCTTCATTCT	GTCTTGCTTGGGCTGGTTTA	42.9	50.0	56.7	58.4	628-1072 bp	1.0	70.4