Hotspot

psbD-trnT-GGU

Genome · 28321-29761 · 1441 bp

Back to Medicago

Pi 0.0199

MarkerSeek score 78.80

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1441
variable_sites: 146
pi: 0.0199
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GAGCAATAGACCGGACCAAC	GTGGTAGAGTAACGCCATGG	55.0	55.0	58.3	58.1	1153-1640 bp	1.0	88.4
psbD-trnT-GGU_p2	GAGCAATAGACCGGACCAAC	TCAGTGGTAGAGTAACGCCA	55.0	50.0	58.3	58.1	1156-1643 bp	1.0	88.2
psbD-trnT-GGU_p3	GAGCAATAGACCGGACCAAC	GGAGCCCCTTTAACTCAGTG	55.0	55.0	58.3	57.9	1170-1657 bp	1.0	88.0
psbD-trnT-GGU_p4	GAGCAATAGACCGGACCAAC	GAGCCCCTTTAACTCAGTGG	55.0	55.0	58.3	57.9	1169-1656 bp	1.0	87.9
psbD-trnT-GGU_p5	AGAGCAATAGACCGGACCAA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.4	58.1	1154-1641 bp	1.0	87.7