Hotspot

trnV-UAC-ndhC

Genome · 10182-10630 · 449 bp

Back to Medicago

Pi 0.0327

MarkerSeek score 78.00

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 449
variable_sites: 80
pi: 0.0327
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	GGCCCTTTTCTAATTGGAGC	50.0	50.0	57.8	56.7	416-621 bp	1.0	80.4
trnV-UAC-ndhC_p2	AGAAGGTCTACGGTTCGAGT	GGCCCTTTTCTAATTGGAGCT	50.0	47.6	57.8	58.3	416-621 bp	1.0	80.3
trnV-UAC-ndhC_p3	AGAAGGTCTACGGTTCGAGT	TGGCCCTTTTCTAATTGGAGC	50.0	47.6	57.8	58.5	417-622 bp	1.0	78.1
trnV-UAC-ndhC_p4	GAAGGTCTACGGTTCGAGTC	GGCCCTTTTCTAATTGGAGC	55.0	50.0	57.5	56.7	415-620 bp	1.0	77.6
trnV-UAC-ndhC_p5	GAAGGTCTACGGTTCGAGTC	GGCCCTTTTCTAATTGGAGCT	55.0	47.6	57.5	58.3	415-620 bp	1.0	77.6