primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	78	490	313-512	0.904762	86.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	78	490	327-526	0.904762	86.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	78	490	326-525	0.904762	86.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	78	490	328-527	0.904762	86.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GCGAATGACGGGAATTGAAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.248789	78	490	365-564	0.904762	86.2
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	AGAAGGTCTACGGTTCGAGT	GGCCCTTTTCTAATTGGAGC	20	20	50.0	50.0	57.8	56.7	0.0	0.0	1.454630	10182	10630	416-621	1.000000	80.4
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	AGAAGGTCTACGGTTCGAGT	GGCCCTTTTCTAATTGGAGCT	20	21	50.0	47.6	57.8	58.3	0.0	0.0	1.457340	10182	10630	416-621	1.000000	80.3
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	AGAAGGTCTACGGTTCGAGT	TGGCCCTTTTCTAATTGGAGC	20	21	50.0	47.6	57.8	58.5	0.0	0.0	1.740706	10182	10630	417-622	1.000000	78.1
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	GAAGGTCTACGGTTCGAGTC	GGCCCTTTTCTAATTGGAGC	20	20	55.0	50.0	57.5	56.7	0.0	0.0	1.798403	10182	10630	415-620	1.000000	77.6
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	GAAGGTCTACGGTTCGAGTC	GGCCCTTTTCTAATTGGAGCT	20	21	55.0	47.6	57.5	58.3	0.0	0.0	1.801112	10182	10630	415-620	1.000000	77.6
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	13649	14860	821-1369	1.000000	90.9
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	13649	14860	829-1377	1.000000	90.4
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	ACCAATTTCGCCATATCCCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.0	57.8	0.0	15.5	0.245117	13649	14860	853-1401	1.000000	90.0
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	13649	14860	861-1409	1.000000	89.5
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	13649	14860	835-1383	1.000000	89.0
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	CGGATAGTGGGAATCGAACC	TTGCTTCTCCTGATGGTTGG	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.355420	24408	25020	623-883	1.000000	89.2
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	CGGATAGTGGGAATCGAACC	GCTTCTCCTGATGGTTGGTC	20	20	55.0	55.0	57.8	58.3	0.0	0.0	0.406401	24408	25020	621-881	1.000000	88.7
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	GCGGATAGTGGGAATCGAAC	TTGCTTCTCCTGATGGTTGG	20	20	55.0	50.0	58.5	57.8	0.0	0.0	0.705974	24408	25020	624-884	1.000000	86.4
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	GGATAGTGGGAATCGAACCC	TTGCTTCTCCTGATGGTTGG	20	20	55.0	50.0	57.5	57.8	19.9	0.0	0.750559	24408	25020	622-882	1.000000	86.0
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	GCGGATAGTGGGAATCGAAC	GCTTCTCCTGATGGTTGGTC	20	20	55.0	55.0	58.5	58.3	0.0	0.0	0.756955	24408	25020	622-882	1.000000	85.9
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	GAGCAATAGACCGGACCAAC	GTGGTAGAGTAACGCCATGG	20	20	55.0	55.0	58.3	58.1	9.5	7.6	0.410916	28321	29761	1153-1640	1.000000	88.4
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	GAGCAATAGACCGGACCAAC	TCAGTGGTAGAGTAACGCCA	20	20	55.0	50.0	58.3	58.1	9.5	0.0	0.433071	28321	29761	1156-1643	1.000000	88.2
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	GAGCAATAGACCGGACCAAC	GGAGCCCCTTTAACTCAGTG	20	20	55.0	55.0	58.3	57.9	9.5	0.0	0.462191	28321	29761	1170-1657	1.000000	88.0
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	GAGCAATAGACCGGACCAAC	GAGCCCCTTTAACTCAGTGG	20	20	55.0	55.0	58.3	57.9	9.5	0.0	0.462191	28321	29761	1169-1656	1.000000	87.9
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	AGAGCAATAGACCGGACCAA	GTGGTAGAGTAACGCCATGG	20	20	50.0	55.0	58.4	58.1	9.5	7.6	0.495564	28321	29761	1154-1641	1.000000	87.7
psbM-petN_p1	psbM-petN	1	AGCTACTGCACTCTTCATTCT	AGTAAGTCTTGCTTGGGCTG	21	20	42.9	50.0	56.7	57.8	0.0	0.0	2.448356	31630	32293	633-1077	1.000000	71.9
psbM-petN_p2	psbM-petN	2	TCGCATTTATAGCTACTGCACT	AGTAAGTCTTGCTTGGGCTG	22	20	40.9	50.0	57.7	57.8	15.5	0.0	2.513207	31630	32293	643-1087	1.000000	71.3
psbM-petN_p3	psbM-petN	3	AGCTACTGCACTCTTCATTCT	ATGGGGAAGAAGTGGACTCT	21	20	42.9	50.0	56.7	57.6	0.0	0.0	2.608091	31630	32293	573-1017	1.000000	71.0
psbM-petN_p4	psbM-petN	4	AGCTACTGCACTCTTCATTCT	TCTTGCTTGGGCTGGTTTAA	21	20	42.9	45.0	56.7	57.6	0.0	0.0	2.630563	31630	32293	627-1071	1.000000	70.5
psbM-petN_p5	psbM-petN	5	AGCTACTGCACTCTTCATTCT	GTCTTGCTTGGGCTGGTTTA	21	20	42.9	50.0	56.7	58.4	0.0	0.0	2.641069	31630	32293	628-1072	1.000000	70.4
atpA-trnR-UCU_p1	atpA-trnR-UCU	1	TCAGGAACAGATGGAACTCT	GGTTCAAATCCTATTGGACGC	20	21	45.0	47.6	55.5	57.3	0.0	0.0	4.222118	51488	51691	232-289	0.952381	56.3
atpA-trnR-UCU_p2	atpA-trnR-UCU	2	AGCTATTCAGGAACAGATGGA	GGTTCAAATCCTATTGGACGC	21	21	42.9	47.6	56.1	57.3	0.0	0.0	4.557432	51488	51691	221-295	1.000000	55.5
atpA-trnR-UCU_p3	atpA-trnR-UCU	3	TCAGGAACAGATGGAACTCTT	GGTTCAAATCCTATTGGACGC	21	21	42.9	47.6	56.3	57.3	0.0	0.0	4.411414	51488	51691	232-289	0.952381	54.8
atpA-trnR-UCU_p4	atpA-trnR-UCU	4	TTCAGGAACAGATGGAACTCT	GGTTCAAATCCTATTGGACGC	21	21	42.9	47.6	56.3	57.3	0.0	0.0	4.411414	51488	51691	233-290	0.952381	54.8
atpA-trnR-UCU_p5	atpA-trnR-UCU	5	TCAGGAACAGATGGAACTCT	TGGATAGGACAGAGGTCTTCT	20	21	45.0	47.6	55.5	56.9	0.0	0.0	4.580932	51488	51691	267-324	0.952381	53.4
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	AATGGGATCCACAGAGACCA	20	20	55.0	50.0	58.1	58.0	16.9	15.2	0.104952	54545	55738	1160-2707	0.857143	85.4
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GAGGTTCGAATCCTTCCGTC	ATGGGATCCACAGAGACCAT	20	20	55.0	50.0	58.1	57.8	16.9	15.2	0.279221	54545	55738	1159-2706	0.857143	84.1
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	TGGAATTGACGAACAACCCA	AATGGGATCCACAGAGACCA	20	20	45.0	50.0	57.6	58.0	13.7	15.2	0.378908	54545	55738	1258-2805	0.809524	81.3
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	GAGGTTCGAATCCTTCCGTC	CAATGGGATCCACAGAGACC	20	20	55.0	55.0	58.1	57.7	16.9	15.2	0.414678	54545	55738	1161-2708	0.809524	81.1
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	GAGGTTCGAATCCTTCCGTC	CCATAACAATTGTCGCAGGC	20	20	55.0	50.0	58.1	57.8	16.9	12.4	0.269407	54545	55738	1745-2457	0.761905	80.3
accD_p1	accD	1	CCCCCGCTCTCTTGTTCTAT	TTGCAACTGCCGGAAATACT	20	20	55.0	45.0	58.9	58.1	0.0	5.0	0.971785	55739	57928	1852-2957	0.714286	72.8
accD_p2	accD	2	CCCCCGCTCTCTTGTTCTAT	AAAGAAGCCATTGCAACTGC	20	20	55.0	45.0	58.9	57.8	0.0	12.8	1.035399	55739	57928	1862-2967	0.714286	72.3
accD_p3	accD	3	CCCCGCTCTCTTGTTCTATT	TTGCAACTGCCGGAAATACT	20	20	50.0	45.0	57.0	58.1	0.0	5.0	1.085779	55739	57928	1851-2956	0.714286	71.9
accD_p4	accD	4	CCCCGCTCTCTTGTTCTATT	AAAGAAGCCATTGCAACTGC	20	20	50.0	45.0	57.0	57.8	0.0	12.8	1.149393	55739	57928	1861-2966	0.714286	71.4
accD_p5	accD	5	AAAGGCGGGTGGTTTTATGT	AGGCCCACTAAAGGAACAAA	20	20	45.0	45.0	58.0	56.9	0.0	0.0	1.067671	55739	57928	1951-2999	0.571429	66.3
rps12-clpP_p1	rps12-clpP	1	GATTCGGTTTGCACCAATCG	AAGCCCACGGAATTGTTGAT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.181593	67986	68854	680-2454	0.809524	82.9
rps12-clpP_p2	rps12-clpP	2	TCCAATGGCTTTTGCTGCTA	AAGCCCACGGAATTGTTGAT	20	20	45.0	45.0	58.1	58.1	0.0	0.0	0.154662	67986	68854	1164-2946	0.714286	79.3
rps12-clpP_p3	rps12-clpP	3	ATTCCAATGGCTTTTGCTGC	AAGCCCACGGAATTGTTGAT	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.180181	67986	68854	1166-2948	0.714286	79.1
rps12-clpP_p4	rps12-clpP	4	TCCAATGGCTTTTGCTGCTA	CTTTTCATGAGGGACGAGCA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.177159	67986	68854	1787-1787	0.047619	40.5
rps12-clpP_p5	rps12-clpP	5	TCCAATGGCTTTTGCTGCTA	TTTTCATGAGGGACGAGCAG	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.177159	67986	68854	1786-1786	0.047619	40.5
clpP_p1	clpP	1	AAAACCCATATAGACGGCGG	AACAGGGTCAGAGGGATCAA	20	20	50.0	50.0	57.8	58.0	13.2	0.0	0.294636	68855	70073	2044-2044	0.047619	39.5
clpP_p2	clpP	2	AAAACCCATATAGACGGCGG	ACAGGGTCAGAGGGATCAAA	20	20	50.0	50.0	57.8	58.0	13.2	0.0	0.294636	68855	70073	2043-2043	0.047619	39.5
clpP_p3	clpP	3	AAAACCCATATAGACGGCGG	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	57.8	58.1	13.2	0.0	0.316822	68855	70073	2076-2076	0.047619	39.4
clpP_p4	clpP	4	AAAACCCATATAGACGGCGG	TGGACAGAAAGTAAACGGCC	20	20	50.0	50.0	57.8	58.1	13.2	0.0	0.362957	68855	70073	1959-1959	0.047619	39.0
clpP_p5	clpP	5	AAAACCCATATAGACGGCGG	AGAACAGGGTCAGAGGGATC	20	20	50.0	55.0	57.8	58.1	13.2	0.0	0.377071	68855	70073	2046-2046	0.047619	38.9
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	CACTTGACTGTCTACGGCTC	ATTGGCGAAGTCGTAGGTTC	20	20	55.0	50.0	58.0	58.0	9.5	2.3	0.021998	84996	85626	774-1203	0.857143	86.1
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	CACTTGACTGTCTACGGCTC	GGCGAAGTCGTAGGTTCAAT	20	20	55.0	50.0	58.0	58.0	9.5	2.3	0.021998	84996	85626	771-1200	0.857143	86.1
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	CACTTGACTGTCTACGGCTC	GAATGGTTAAAGCGCCCAAC	20	20	55.0	50.0	58.0	58.0	9.5	2.3	0.025228	84996	85626	799-1228	0.857143	86.1
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	CACTTGACTGTCTACGGCTC	TATCGATTGCCTTTGCGTGA	20	20	55.0	45.0	58.0	58.0	9.5	1.7	0.033166	84996	85626	1112-1553	0.761905	82.2
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	CACTTGACTGTCTACGGCTC	TATCATTGCTGGGTCCAACG	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.052985	84996	85626	1255-1696	0.571429	72.6
ycf2_p1	ycf2	1	AAGAGAATTGGCGGAATCCC	AATTCGCTTGGATCCATCCC	20	20	50.0	50.0	57.9	58.0	0.0	17.1	0.071710	87868	87987	527-800	0.619048	76.2
ycf2_p2	ycf2	2	GAGAAGAAGAAGCGCAGGAA	AATTCGCTTGGATCCATCCC	20	20	50.0	50.0	57.9	58.0	11.5	17.1	0.099172	87868	87987	588-861	0.619048	76.0
ycf2_p3	ycf2	3	AAGAGAAGAAGAAGCGCAGG	AATTCGCTTGGATCCATCCC	20	20	50.0	50.0	57.9	58.0	11.5	17.1	0.104970	87868	87987	590-863	0.619048	75.9
ycf2_p4	ycf2	4	CGATCAAGAGAATTGGCGGA	AATTCGCTTGGATCCATCCC	20	20	50.0	50.0	58.1	58.0	0.0	17.1	0.073143	87868	87987	562-805	0.571429	72.1
ycf2_p5	ycf2	5	ATATGCGGGAAGGTCCTGTA	AATTCGCTTGGATCCATCCC	20	20	50.0	50.0	57.9	58.0	5.1	17.1	0.110228	87868	87987	433-484	0.238095	60.6
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.7	58.2	7.8	0.0	0.485056	98587	98895	402-621	1.000000	88.1
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.496177	98587	98895	424-643	1.000000	88.0
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	AAGTCGTAACAAGGTAGCCG	GGCACAACGACGCAATTATC	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.548742	98587	98895	411-630	1.000000	87.6
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	AACAAGGTAGCCGTACTGGA	GGCACAACGACGCAATTATC	20	20	50.0	50.0	58.4	58.2	7.8	0.0	0.553655	98587	98895	404-623	1.000000	87.6
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	ACAAGGTAGCCGTACTGGAA	GGCACAACGACGCAATTATC	20	20	50.0	50.0	58.4	58.2	7.8	0.0	0.553655	98587	98895	403-622	1.000000	87.6
ycf1-rps15_p1	ycf1-rps15	1	TGTATCCTCGATTGAAAGATT	TCAACTGATCCGGGGTTTTC	21	20	33.3	50.0	52.4	57.8	1.4	0.0	6.830728	110870	111293	326-650	0.952381	50.1
ycf1-rps15_p2	ycf1-rps15	2	TGTATCCTCGATTGAAAGATT	CAACTGATCCGGGGTTTTCT	21	20	33.3	50.0	52.4	57.8	1.4	0.0	6.836899	110870	111293	325-649	0.952381	50.1
ycf1-rps15_p3	ycf1-rps15	3	ATGTATCCTCGATTGAAAGAT	TCAACTGATCCGGGGTTTTC	21	20	33.3	50.0	52.1	57.8	0.0	0.0	7.069045	110870	111293	327-651	0.952381	50.1
ycf1-rps15_p4	ycf1-rps15	4	ATGTATCCTCGATTGAAAGAT	CAACTGATCCGGGGTTTTCT	21	20	33.3	50.0	52.1	57.8	0.0	0.0	7.075216	110870	111293	326-650	0.952381	50.1
ycf1-rps15_p5	ycf1-rps15	5	TGTATCCTCGATTGAAAGATT	ACTGATCCGGGGTTTTCTTC	21	20	33.3	50.0	52.4	57.5	1.4	0.0	7.128201	110870	111293	323-647	0.952381	50.1