Hotspot

ycf1

SSC · 122941-128301 · 5361 bp

Back to Meconopsis

Pi 0.0293

MarkerSeek score 66.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5361
variable_sites: 650
pi: 0.0293
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCTCCGTCTTGTTGCTCC	AACAAGAATGCCTGCCTGAA	50.0	45.0	58.1	58.0	384-396 bp	1.0	90.9
ycf1_p2	CCAATCCTCATTCCACTCCG	AACAAGAATGCCTGCCTGAA	55.0	45.0	58.0	58.0	974-986 bp	1.0	89.7
ycf1_p3	CAATCCTCATTCCACTCCGG	AACAAGAATGCCTGCCTGAA	55.0	45.0	58.0	58.0	973-985 bp	0.8	81.2
ycf1_p4	CCTCGGCCATTTCTTCTTCA	AACAAGAATGCCTGCCTGAA	50.0	45.0	57.9	58.0	408-417 bp	0.666667	72.5
ycf1_p5	CTTGCGAATAACGGAGGACA	AACAAGAATGCCTGCCTGAA	50.0	45.0	58.0	58.0	528-540 bp	0.466667	67.2